2024 Abstracts

Authors: Elizabeth M J Allen, Steven P Allen, Henrik C A Odeen. Mentors: Steven P Allen. Insitution: Brigham Young University. BackgroundThe focus of this project was creating phantoms with customizable attenuation and stiffness for visualizing histotripsy lesions created with magnetic resonance guided focused ultrasound surgery (MRgFUS). Having phantoms with varying mechanical and acoustic properties is important because they affect cavitation and allow for testing of different histotripsy mechanisms. Creating a tunable phantom with red blood cells (RBCs) spread throughout it is valuable because it allows us to visualize High Intensity Focused Ultrasound (HIFU) lesions from MRgFUS in three dimensions throughout the gel.Materials and MethodsWe produced gels with tunable acoustic and mechanical properties by mixing 5 different ratios of evaporated milk and deionized water with 5 different ratios of agarose powder to create 25 different gels.Gel attenuation was measured using a through transmission setup and Young’s Modulus was obtained using a tensile tester in compression. The MR properties, including T1, T2, and T2* of each gel were also measured prior to creating histotripsy lesions.ResultsIn general, the agarose gels had an increase in attenuation as the amount of evaporated milk increased, and an increase in mechanical stiffness as the amount of agarose powder increased. They also provided excellent lesioning contrast for visualizing MRgFUS lesions.ConclusionsWe successfully created a series of tunable gels for visualizing MRgFUS lesions. These gels are also useful for characterizing ultrasound transducers and can be used to test emerging MRgFUS technology as it becomes more widely used and is further developed.

Authors: Cate Miller, Tyler Free, Brad Bundy. Mentors: Brad Bundy. Insitution: Brigham Young University. Diagnostics are an essential tool in the treatment path for many different types of cancer. They are used to determine the progression of cancer treatment and are critical to increase survival rates. The most common diagnostics are often too expensive or sophisticated to be used on a widespread scale. Using a cell-free protein synthesis system to create a biosensor as a diagnostic can drastically decrease costs, increase convenience, and increase survival rates. We previously engineered low-cost CFPS systems to detect glutamine which would be useful for low-cost biosensing of clinically relevant glutamine concentrations. This work reports our progress in optimizing the CFPS glutamine biosensor for improved performance.

Authors: Garrett Johnson, Tyler Free, Brad Bundy. Mentors: Brad Bundy. Insitution: Brigham Young University. Acute Lymphoblastic Leukemia is the most common form of childhood cancer. Asparaginase treatment has greatly improved survival rates to 80%. Survival rates increase by an additional 10% when diagnostic treatment monitoring is used to guide treatment administration. Cell-free protein synthesis is a technology capable of detecting Asparaginase in human serum. However, RNases from human samples destroy CFPS activity. RNase inhibitor can be produced at a low cost by the CFPS system to rescue in-vitro protein synthesis activity to enable biosensing. This work reports our work toward developing an at home Asparaginase diagnostic that would enable personalized dosing of Asparaginase and further improve survival rates of Acute Lymphoblastic Leukemia.

Authors: Alex Montgomery, Mason Gordon. Mentors: Eddy Cadet. Insitution: Utah Valley University. TRACE METAL CONCENTRATIONS OF VARIOUS LAND USE TYPES SURROUNDING UTAH LAKEMason Gordon, Alex MontgomeryKeywords: Trace metals, Soil, Land use, Utah LakeUtah Lake has a history of anthropogenic impacts that have resulted in the accumulation of trace metals (TMs) in the sediments of this region. Previous studies have evaluated the water and saturated soils, but have not provided a complete picture of the human impact on upland soils. Some pollutants may be contained in unsaturated soils and never enter the water due to the inherent soil characteristics and chemical properties of the TMs. Elevated concentrations of TMs in the environment pose hazards to the ecosystem and local residents. These impacts can be better understood by evaluating TMs in unsaturated upland soils. In this study a comparison of TMs in saturated wetland and unsaturated upland sediments, as related to anthropogenic sources, was completed. This study analyzed the types and concentrations of TMs to understand their mobility throughout the ecosystem. 52 core samples were collected from the saturated and unsaturated soils in eight sites. These sites represent recently developed areas (New Dev), more established areas (Mid Dev- those that have been developed over five years), recreational areas, mining areas, industrial areas, agricultural areas, and a wastewater treatment plant site. The soil samples were dried, ground, sieved, acid digested, and analyzed in the ICP-OES for TM (As, Pb, Cu, Cr, Cd, and Zn) content. Preliminary results show that TM concentrations in upland sediments were higher than those in wetland sediments. Cr levels in industry and Mid Dev are 43.0 ppm and 47 ppm, respectively. Saturated sediments at the same sites revealed Cr levels of 23 ppm and 21 ppm, respectively. This indicates that TMs are contained in unsaturated sediments. Of the observed land use types, Mid Dev is the most impacted, having the highest elevations of TM levels on average. Cd concentrations exceeded background levels in both New Dev (.97 ppm) and Mid Dev (.83 ppm). This is indicative of anthropogenic impact, as developed areas have higher TM content. The statements of this study will provide information to regulatory authorities in order to create policy to improve human health.

Authors: Gabe Snow, Joseph Ward. Mentors: Cammy Peterson. Insitution: Brigham Young University. Unmanned aerial system (UAS) research is fast becoming an important area of development in commercial and military applications. As UAS become more prevalent commercially and recreationally, there is a growing need to accurately track large numbers of these aircraft. This is particularly important in compact urban environments where potential flight paths are limited. Our research team at BYU is developing the Local Air Traffic Information System (LATIS), allowing multiple radar ground stations to communicate and track UAS across multiple fields of view. One major component of this system is Recursive Random Sample Consensus (R-RANSAC)---an algorithm used to correlate and combine the data from multiple sources. The process a ground station uses for calibration is to collect Real-Time Kinematic Global Positioning System (RTK GPS) coordinates of a friendly UAS as it passes through the field of view of a station's radar. R-RANSAC is then used to a) filter noise from the raw radar data, and b) identify "tracks," or paths which UAS have followed, using temporal and spatial proximity. The Orthogonal Procrustes Problem then provides a method to rotate data from the local radar frame to the global frame. These steps can be done live or with recorded data. Following this, the calibrated radar uses R-RANSAC to filter data and identify passing UAS with high accuracy. Our contributions to the project are developing communication software, refining R-RANSAC, and helping to implement the whole system in flight experiments. We are continuing to work on analyzing the data taken from flight tests and publish the improvements of this system compared to existing methods.

Authors: Jacob M Wendt. Mentors: Brandon Ro. Insitution: Utah Valley University. This research delves into the transformation of urbanization over the past century, triggered by the influence of modern architectural concepts, and the potential ramifications for mental health. The primary objective is to investigate the neurological effects of specific urban layouts, with a particular emphasis on safety, social interaction, and aesthetic appeal, to gain insight into the human mind's urban preferences. A survey will be designed to capture subjective preferences in which respondents choose one of two anonymous center streets that are subjectively safe and attractive. Participants will favor the traditional street layout over the contemporary one, suggesting a preference for design associated with enhanced safety, aesthetics, and social appeal, affirming the statement by Allen Jacobs that optimal streets have paramount desirability. In parallel, eye-tracking software is utilized to objectively analyze subconscious neurological responses to diverse urban layouts and elements, providing a more scientific perspective on human perceptions of urban environments by revealing more balanced heat maps, and signifying a focus on aesthetic elements and reduced attention to potential hazards. The combination of survey and eye-tracking data presents a comprehensive argument in favor of traditional urban planning principles and expected to underscore the prevalent preference for traditional urban designs over contemporary alternatives. In conclusion, this research sheds light on the enduring human desire for both safety and aesthetic beauty in urban environments. Anonymous survey data ranging from 50 to 100 participants, coupled with eye-tracking simulations indicating balanced heat maps, highlights the potential of traditional design to cultivate healthier and more harmonious communities. Future research may delve into physiological measures, such as heart rate monitoring, to further validate the stress-reducing benefits of traditional urban planning. The findings from this survey are expected to provide actionable insights that can inform urban planning decisions and strategies that foster improved mental wellness and community well-being.

Authors: Aubrilin Johnson. Mentors: Yu Ya Liang. Insitution: Utah Valley University. Drought stress negatively affects the growth, survival, and production of crops. Developing drought-tolerant cultivars is an important strategy for maintaining crop yields under undesirable water conditions. Tomatoes are an important crop both grown worldwide and here in Utah. Improving drought tolerance in tomato plants can increase yearly yields and reduce the amount of crops lost during the periodic droughts that Utah has. A way to develop drought-tolerant tomatoes may be through identifying and utilizing genes that enhance survival and growth rates under stressful water conditions. In this study, we will use two varieties of tomato, including drought-tolerant wild species, and susceptible commercial varieties. According to previous studies, drought during late the vegetative stage will significantly reduce the yield, therefore this study will focus on that. Using RNA-seq to find the difference in gene expression between the control and drought groups. Identifying differences will facilitate in finding candidate genes that may have a potential role in drought resistance. Then take the top cultivar and find the specific drought-resistant gene, which can then be used by Utah tomato growers to yield more resistant crops.

Authors: Ishmael Elliott Molina-Zepeda, Brandt Jones, Myke Madsen, Douglas Sborov, Brian Avery, Nicola J. Camp . Mentors: Nicola J. Camp. Insitution: University of Utah. Multiple Myeloma (MM) is a malignancy of plasma cells and one of the more common hematological malignancies (6.3/100,000 new cases/year). Although treatments have improved, most patients fail their first line of treatment and ultimately do not survive beyond 5 years. Identifying patients at high risk of failing treatment early is a critical need. SPECTRA is a statistical technique developed by the Camp Lab to characterize global gene expression (the transcriptome) by representing it as multiple quantitative tumor variables. Spectra variables allow gene expression to be incorporated into predictive modeling to identify high-risk groups.Transcriptome data for myeloma cells was available from 768 patients in the international CoMMpass study where 39 spectra variables were derived. Each patient has a value for each of the 39 variables (their spectra “barcode”); patients can be compared for each bar in the barcode. Predictive modeling using spectra variables was successful in identifying risk groups for time to treatment failure, such that a patient’s tumor transcriptome can be used to predict whether they are at high risk of having their treatment fail earlier.To replicate the CoMMpass data findings, we collect and process local biological samples from MM patients at the Huntsman Cancer Institute (HCI). We collect bone marrow samples, which are then cell-sorted to identify tumor (CD138+) cells. RNA is extracted from these cells and sequenced to generate transcriptome data. Then the spectra barcode is calculated.Utilizing the SPECTRA technique provides a more complete understanding of MM by better characterizing the tumor. Each spectra is a tumor characteristic. Our future research includes an investigation of whether inherited variations (in normal DNA from saliva or whole blood) are associated with the transcriptome risk score. We are also pursuing the SPECTRA technique in several other cancers.

Authors: Sehi Kim, Naomi Rapier-Sharman, Michael Told. Mentors: Brett Pickett. Insitution: Brigham Young University. Systemic Lupus Erythematosus is an autoimmune disease that produces autoantibodies affecting various body regions, including skin, joints, kidneys, brain, aerosol surfaces, blood vessels, etc., resulting in damaging organs and tissue. Patients commonly experience an elevated risk of bleeding or blood clotting, joint stiffness, pain, fatigue, and depression.Our study involved the collection of RNA seq data of B cells of both SLE patients and healthy people from the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) database. Subsequently, we employed the Automated Reproducible MOdular Workflow for preprocessing and differential analysis of RNA-seq data (ARMOR) workflow. The differentially expressed genes identified by ARMOR were then analyzed using SPIA (Signaling Pathway Impact Analysis) algorithm to find the pathways associated with lupus. We further utilized the Pathways2Targets algorithm to predict potential lupus treatments based on known protein-drug interactions.In our study on lupus patients, analysis using ARMOR, SPIA, and Pathways2Targets identified 10,000 differentially expressed genes and revealed their modulated pathways, providing insights into molecular cascades in lupus. Furthermore, we identified potential drug targets, finding the way for therapeutic interventions that ultimately led to the discovery of new drug treatments. We anticipate that our findings could be utilized for the benefit of lupus patients, further advancing personalized medicine strategies, holding promise for improving the quality of life for individuals grappling with this complex autoimmune disorder.

Authors: Trenton M. Gibson, Brett E Pickett. Mentors: Brett E Pickett. Insitution: Brigham Young University. Lynch Syndrome, formerly known as Hereditary Non-Polyposis Colorectal Cancer, is an autosomal dominant condition characterized by non-functional DNA mismatch repair genes, leading to mismatch repair deficiency (dMMR). Individuals with dMMR have an increased risk of developing cancerous tumors with microsatellite instability (MSI). The aim of this study was to investigate significant perturbations in gene expression among different dMMR variants within Lynch Syndrome, specifically focusing on variants in the PMS2, MLH1, and MSH2 loci. We obtained 55 public RNA-seq human colorectal tumor samples with specific germline dMMR mutations and 20 samples of healthy colorectal epithelium from the GENE Expression Omnibus (GEO). These samples were subjected to a robust RNA-seq data preprocessing and analytical workflow, which involved quality control, trimming, read pseudomapping and quantification using Salmon, identification of significant differentially expressed genes using edgeR, and gene ontology enrichment analysis using Camera. Signaling pathway impact analysis (SPIA) was employed to assess gene pathway perturbations. Our results revealed that tumor samples from individuals with germline mutations in the PMS2 MMR gene exhibited transcriptomic profiles indicating upregulation in pathways related to intrinsic and extrinsic prothrombin activation, fibrinolysis, and uPA/uPAR-mediated signaling, in comparison to other tumor and healthy samples. Previous research has established a correlation between these pathways and tumor growth, invasiveness, and metastasis. These findings provide a foundation for further research to explore the interactions between PMS2 and these pathways in influencing tumor development.

Authors: Kiersten Gardner, Hannah Duffy, Abbey Blair, Nicholas Ashton, Porter Stulce, Dustin Williams. Mentors: Dustin Williams. Insitution: University of Utah. Transdermal antiseptic products as a method to decrease skin bioburden prior to surgeryKiersten Gardner(1,2), Hannah Duffy(1,2), Abbey Blair(2), Nicholas Ashton(2), Porter Stulce1(2), Dustin Williams(1,2,3,4)1 Department of Biomedical Engineering, University of Utah, Salt Lake City, UT2 Department of Orthopaedics, University of Utah, Salt Lake City, UT3 Department of Pathology, University of Utah, Salt Lake City, UT4 Department of Physical Medicine and Rehabilitation, Uniformed Services University, Bethesda MDIntroduction: Preoperative skin preparation (PSP) kits are used before surgery to prevent surgical site infection (SSI). These kits consist of alternating scrubs of alcohol and Chlorohexidine Gluconate (CHG) or Povidone Iodine (PVP-I). Transdermal antiseptic products like Ioban, Tegaderm, and Surgiclear are also used clinically to eradicate skin bacteria by releasing antiseptic over time. Despite these precautions, infections often occur, usually stemming from a patient’s endogenous skin flora. The bacteria causing SSI reside deep in dermal sweat glands and hair follicles, untouched by traditional PSP. To eliminate these bacteria, antiseptics must diffuse deeper into the skin at concentrations above the minimum inhibitory concentration (MIC). We screened FDA-approved topical antiseptic products using a modified Kirby Bauer Assay to assess bacterial kill over time. We hypothesized that extended use of topical antiseptic products prior to surgery would kill deep-dwelling skin bacteria. We tested the extended use of these products on pig backs in conjunction with a CHG PSP to evaluate remaining bioburden. Methods:We determined the minimum inhibitory concentrations (MIC) for CHG and PVP-I against three common skin bacterial strains. We then took 6 mm biopsy punches of Tegaderm, Surgiclear, and Ioban and placed them on fresh bacterial lawns daily for 3 days. We measured the zones of inhibition (ZOI). Lastly, we applied the products to the backs of 4 Yorkshire pigs (n=4 products per test). After 48 h, we removed the products, performed a PSP- CHG scrub, and excised skin sections underneath the products. We homogenized the skin and quantified colony forming units (CFU)/g tissue. We analyzed the outcomes statistically using a mixed effects linear regression to determine significance.Results:The average MIC values for CHG and PVP-I were 2 and 2,000 ug/mL for Staphylococcus aureus, respectively. The average 24 h ZOIs for S. aureus were 7.9, 22.4, and 10.1 mm for Ioban, Tegaderm, and Surgiclear, respectively. The average log10 reduction for the CHG PSP, Ioban, Tegaderm, and Sugiclear were 1.87 ± 0.232, 1.65 ± 0.192, 1.69 ± 0.222, and 1.77 ± 0.316 CFU/cm2, respectively. The p values between the CHG PSP and Ioban, Tegarderm, and Surgiclear were 0.491, 0.572, and 0.746, respectively. Discussion:The benchtop data indicated adequate antiseptic diffusion and kill. In the porcine model, however; the presence of any of the products resulted in a statistically insignificant log reduction. Extended use of FDA-approved transdermal antiseptic products does not appear to decrease the skin’s bioburden. It appears that the products are not releasing high enough concentrations of the antiseptic to kill bacteria.

Authors: Diya Garg, Chadi Hage Chehade, Yeonjung Jo, Georges Gebrael, Nishita Tripathi, Beverly Chigarira, Arshit Narang, Vinay Matthew Thomas, Gliceida Galarza Fortuna, Patrick Campbell, Clara Tandar, Ayana Srivastava, Nicolas Sayegh, Sumati Gupta, Benjamin L. Maughan, Soumyajit Roy, Neeraj Agarwal, Umang Swami. Mentors: Neeraj Agarwal. Insitution: University of Utah. Trends in tumor NGS genomic testing at diagnosis of metastatic prostate cancer (mPC) and urothelial carcinoma (mUC) Diya Garg1, Chadi Hage Chehade1, Yeonjung Jo1, Georges Gebrael1, Nishita Tripathi1, Beverly Chigarira1, Arshit Narang1, Vinay Matthew Thomas1, Gliceida Galarza Fortuna1, Patrick Campbell1, Clara Tandar1, Ayana Srivastava1, Nicolas Sayegh1, Sumati Gupta1, Benjamin L. Maughan1, Soumyajit Roy1, Neeraj Agarwal1, Umang Swami1 1Huntsman Cancer Institute at the University of Utah, Salt Lake City, UT; Objectives: Next-generation sequencing (NGS) can allow receipt of approved targeted therapies and recruitment into clinical trials and aid with prognostication and response to therapy. In mPC, the presence of homologous recombination repair (HRR) alterations can render patients (pts) eligible for PARP inhibitor therapies [PMID: 37442702]. In mUC, genomic alterations in FGFR3 or FGFR2 can make them eligible for erdafitinib [PMID: 31340094]. Multiple agents have tumor agnostic approvals for molecular specific alterations. Herein, we aim to analyze the current trends in NGS testing in both mPC and mUC. Methods: A de-identified nationwide Flatiron Health Electronic Health Record (EHR)-derived database was used to extract pt-level data. Eligibility: pts diagnosed with mPC or mUC and receiving treatment for their disease between March 2015 and December 2022. The NGS test was considered performed at diagnosis if completed within a year of the initial metastatic disease diagnosis. A two-sided 95% confidence interval (CI) was calculated using Clopper and Pearson method. The analysis was done using R version 4.2.3. Results: The study included 11,936 and 6,340 pts in the mPC and mUC cohorts, respectively. For mPC, the rate of NGS testing among men in 2015 was only 1.3% (95% CI 0.7-2.1%). This rate steadily increased, plateauing between 2021 and 2022 at 28% (95% CI 25.8-30.3%) and 27.1% (95% CI 24.5-29.8%), respectively. NGS testing was mainly performed on samples from primary prostate tissue (46.1%), followed by blood (32.8%) and saliva (0.1%) or from any of these 3 sites (2.9%). In the mUC cohort, the rate of testing among pts in 2015 was 6.7% (95% CI 4.8-8.9%). However, by 2021 and 2022, the rates had improved, reaching 46.8% (95% CI 43.5 – 50.2%) and 46.5% (95% CI 42.5-50.5%), respectively. Samples for NGS testing were mainly obtained from primary bladder tissue (72.8%), followed by blood (15.6%), or from both sites (0.2%). The sample source for testing was unknown in 18.1% and 11.4% of cases in mPC and mUC, respectively. Conclusions: In this large real-world analysis, we show that while the rate of NGS testing has improved over time, the majority of pts do not undergo NGS testing in both mPC and mUC cohorts. Addressing barriers to NGS testing remains an unmet need. Incorporating tumor genomic testing into the standard of care management can optimize treatment selection, prognostication, and access to clinical trials.

Authors: Mikayla Kimball, Noah Bezzant, Ashley Allan, Josh Sponbeck. Mentors: Brent Feland. Insitution: Brigham Young University. Ultrasonic analysis of patellar tendon thickness in active older athletesBACKGROUND: Recent research has suggested that patellar tendon loading through exercise and resistance training can help maintain and increase patellar tendon thickness in older adults. Limited research exists that identifies the average thickness of patellar tendons in younger athletes, however, it is unknown if this thickness remains or is maintained in older adult athletes who have maintained a very active lifestyle.PURPOSE: This study aimed to determine how gender correlates to patellar tendon thickness in the proximal and middle patellar tendon of active older athletes participating in sporting events at the Huntsman World Senior Games.METHODS: Data was collected from 59 volunteers (participants in the Huntsman WorldSenior Games) in St. George, Utah, 2022. All subjects (34 females: mean age = 61.09 ± 7.00 yrs, Ht = 162.41 ± 25.73 cm, Wt= 66.29 ±11.38 kg; 25 males: mean age = 68.68 ± 7.03 yrs, Ht = 178.21 ± 8.63 cm, Wt= 84.42±10.90 kg) signed an approved consent form and then sat on a treatment table with their legs relaxed and dangling off. The probe was placed vertically below the kneecap and an ultrasonic image was taken. Each image showed a small section of the patellar for reference. Each ultrasonic measurement showed the middle and proximal thickness of the patellar tendon. ANALYSIS: All data were analyzed using JMP ver16.2 with a stepwise multiple regression analysis to determine the effect of age, height, wt and gender on patellar tendon thickness. A sex*location mixed model was used to determine differences in middle and proximal thickness between gender. Data were normally distributed, not requiring transformation.RESULTS & CONCLUSIONS: Proximal tendon measurements were thicker than middle tendon measurements on both sides (p=0.0001). There was no significant difference either proximal tendon thickness (p=0.9323) or middle tendon thickness (p= 0.3993) between left and right sides. No significant difference between male and female tendon thickness at either location (p=0.7700). Proximal tendon thickness was greater and this has been found to be greater in younger athletes with a history of patellar tendinopathy. Aging athletes may also have a history of knee pain episodes that could have contributed to this finding. The lack of gender differences in thickness measures was surprising, but may be a result of the level of activity of senior athletes. In the future studies should look to compare active vs non-active aging athletes, more specific age range differences, and how knee replacements and other injuries affect patellar tendon thickness.

Authors: Rebekah Victoria Still. Mentors: Alexandra Giannell. Insitution: Utah Valley University. We all experience panic. For many people it is a rare experience, while for those diagnosed with panic disorders, it can be a regular and debilitating occurrence. Oftentimes, it’s embarrassing and difficult for those living with such a disorder to explain to friends, family, coworkers, supervisors, and peers what they’re feeling and why it affects their lives so thoroughly. In this project, I approached various strangers to ask them about their experiences with panic in an effort to develop a unique and universal language, which would enable viewers to better understand panic and open an empathetic dialogue between those with such a disorder and their loved ones.Based on the answers I received, I was able to sort the data and create multiple visual recipes which I used to develop a series of preliminary works. With each rendition, I asked for feedback from those with and without panic disorders, so as to assess the effectiveness of my color palette, symbology, and mark making techniques. Through this process of creation and criticism, I arrived at a composition which successfully encapsulates the feelings, sounds, and appearance of panic.As someone who lives with PTSD, I believe that it’s important to foster empathy for those around us and earn how to effectively communicate our feelings. My objective is that through this work, people who previously didn’t have the words to discuss their mental health will be able to use this piece to start an open and honest conversation with their loved ones. Furthermore, by using a universal, visual language, those who don’t have panic disorders will be able to begin the process of opening their minds and hearts to understand the people who do. In this sense, my final painting is not an answer, but a question meant to inspire further research and exploration.

Authors: Isaac Stubbs, Skyler Russell, Melissa Blotter, Maxwell Holmes. Mentors: Ryley Parrish. Insitution: Brigham Young University. Status Epilepticus (SE) is a severe medical condition marked by continuous seizures lasting over 5 minutes. When SE becomes resistant to anticonvulsant drugs, the condition is known as Refractory Status Epilepticus (RSE), which lacks effective treatments and has a mortality rate of 38%. RSE lacks effective treatments partially due to our limited understanding of the mechanisms that lead to patient drug resistance to commonly used anticonvulsants. This study aims to address this knowledge gap in two pivotal ways.First, we have employed a high-density multi-electrode array (HD-MEA) with acute mouse brain slices to better understand RSE propagation patterns and various seizure states with unparalleled spatial precision. The HD-MEA allows us to record from the entire brain slice with 4096 electrodes sampling electrophysiological activity at every 60 micrometers for many hours at a time. Our data demonstrates that different seizure states, such as phasic seizure-like events, short duration epileptic discharges, or RSE itself, occur within both the same brain region and in different brain regions simultaneously. With our novel data visualization software, we can visualize the unique propagation of this phenomenon. These findings indicate that RSE might be a progressive event, challenging conventional understanding of RSE. Second, we are currently exploring a potential pharmacoresistance mechanism that may contribute to the patient entering RSE, which suggests that changes in the chloride reversal potential may lead to a phenomenon known as depolarizing GABA. Depolarizing GABA may negate the effectiveness of the currently used antiepileptic drugs that rely on standard physiological chloride conductance to effectively limit seizure activity. We are studying this drug resistant mechanism with the HD-MEA by introducing anticonvulsant drugs to acute mouse brain slices during the evolution of RSE to locate a critical point at which the slice becomes resistant to these compounds.We hope this study will illuminate the complexities of RSE by revealing its progressive nature and drug resistant properties.

Authors: A. Kalani Brubaker. Mentors: Nathan Crane. Insitution: Brigham Young University. Binder Jetting is an exciting form of Additive Manufacturing in which a binding agent is selectively deposited on layers of powder to bind the powder together and create a 3D printed part. It is notable for its wide range of materials, energy efficiency, and built-in supports. Much research has been done on how to achieve desirable properties of binder-jetted parts and post-processing, but the fundamental principles of binder-powder interaction and the effects of changing printing parameters are still not fully understood. This research aims to increase that understanding. Each binder-jetted part begins by depositing individual binder droplets in a straight line, and the binder-powder droplets coalesce to form a single line. Adjacent lines are then printed to form a cross-section of the part, the next layer of powder is applied, and the process continues until a 3D part is printed. If the spacing between the droplets is too large or the inter-arrival time too short, the droplets form spheres on the surface of the powder (a phenomenon called balling), and a continuous line is not formed. This research determines how continuous line formation is affected by droplet spacing, inter-arrival time, and controlled misting of the powder bed prior to printing. To examine the effects of these parameters, lines were printed in dry and prewetted powder beds while varying the spacing and inter-arrival time between droplets. The beds were then examined using an optical microscope to determine whether continuous lines were formed. The results were recorded on a graph of inter-arrival time versus droplet spacing, and the “boundary line” for successful line formation was found for each bed. The results showed that the boundary on the prewetted data exhibited a different relationship than dry powder beds, suggesting that a different binder-powder principle may dominate after prewetting. The prewetting also shifted the boundary upwards, making it possible to form continuous lines at higher droplet spacings and shorter inter-arrival times. This increased ability to form continuous lines has the potential to significantly increase the throughput of binder-jetted parts.

Authors: Eric Burton. Mentors: Brandon Ro. Insitution: Utah Valley University. Which approach creates more interesting and beautiful buildings: Those who utilize ‘form following function’, or ‘function following form’? When an architect designs only using form following function, the building can have an excess of the utilitarian, resulting in a building that is very useful but can suffer in its overall design aesthetic, lacking attention to beauty. When the opposite occurs, the building can be an incredible work of art, but perhaps suffer in its usage. For an architect to be successful, there needs to be a balance of both methods.In the field of architecture, many architects and designers follow the adage ‘form ever follows function’. While the purpose of this research project is not to discredit this philosophy, the goal is to prove that the other side of the architectural coin, so to speak, has value and that there are multiple philosophies to consider.The research methodology for this project was comparing the buildings of architects that utilize differing philosophies with various building types, and utilizing eye tracking software to help determine which philosophy is more engaging, and beautiful to the average person. The results show that function following form typically produces buildings that are considered more pleasing to the eye, when it comes to the building facades that are evaluated. Building with form in mind first will allow for the designs to better fit their perceived building typology. There is worth at looking into different perspectives. When one is approaching design, they should be willing to implement multiple methods to achieve the best version of their building. Function following form is one method that can help us attain a more beautiful building experience that will impact the world for generations to come.

Authors: Harmony Newbrough. Insitution: Utah Valley University. During the time of Ancient Rome, there were many sarcophagi that were created that had faceless portraits, and it could have been for a number of reasons, whether it be financial or personal. These unfinished portrait faces have puzzled archaeologists and historians alike in trying to find a concrete answer as to why there are so many of them,