Presenter: Trevor Millar
Authors: Trevor Millar, Ernie Vilela, T. Ogden
Faculty Advisor: T. Heath Ogden
Institution: Utah Valley University
Next Generation Sequencing (NGS) technologies are regarded as a highly accurate and cost effective method of DNA sequencing. Anchored Hybrid Enrichment (AHE) has been used across different taxonomic groups for phylogenomic estimation, including mayflies (Miller et al. 2018, Ogden et al. 2019). These methods have led to the largest and most robust analyses for Ephemeroptera to date. The biggest obstacle posed by NGS and AHE is that these analyses produce immense amounts of data that require processing before a data set can be used in a phylogenetic analysis. To compound this issue, many of the software packages suggested in recent analyses are not readily accessible to most users, such as in Breinholt et al. 2018. The purpose of this work is to publish a fully reproducible workflow, from raw NGS data to a phylogenetic tree, using readily available software and well documented methods.