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The effects of aerated & non-aerated reverse-osmosis water & tap water on lettuce phenotypes

December 30, 0020 12:00 AM
Tyler Hacking, Jonathan Wasden and Dr. Michael Stevens (Utah Valley University)
Faculty Advisor: Stevens, Michael (Science, Biology)

Abstract:

Plant growth can be affected by both genes and the environment. To test genetic effects on growth we used two varieties of lettuce. To test environmental effects on growth, we used four different water treatments. We were interested in the main effects of genes and the environment and also in their interaction. In other words, is the effect of water treatment different across two lettuce varieties? Two varieties of Lactuca sativa were cultivated in a growth chamber in pots using vermiculite as the growth medium. The two varieties were treated with four types of water: tap, tap-aerated, reverse osmosis, and reverse-osmosis-aerated throughout the experiment. Aeration was accomplished using both surface aeration (waterfall and vortex) and sub-surface aeration using submerged waterstones. All plants were fertilized using 8-15-36 (N-P-K) water-soluble lettuce fertilizer powder plus trace minerals by Greenway Biotech, Inc. The plants were harvested and dried to a constant weight for analysis of biomass. We observed varietal differences in growth with the Buttercrunch variety producing 145% more biomass than the Butterhead variety (p < 0.001). The water treatments affected growth, with the plants watered with tap water producing 31% more biomass than the plants watered with reverse-osmosis water (p < 0.001). The other two water treatments (tap-aerated and reverse-osmosis-aerated) were intermediate between the tap-watered plants and the reverse-osmosis-watered plants in terms of biomass. Finally, we observed a variety-by-environment interaction such that the Buttercrunch showed a marked response to water treatment whereas the Butterhead was only minimally affected by water treatment (p < 0.001). The Buttercrunch produced more biomass than the Butterhead because of its shorter life cycle. By the end of the experiment, the Buttercrunch plants were already bolting and producing flowers. This could also explain why the Buttercrunch was more responsive to differences in water treatments.
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Amaebae in warming soils : The interactive effects of protozoan predation pressure and environmental factors on Nitrogen Cycling in soils under warming conditions

December 30, 0020 12:00 AM
Dodge, Reagan; Backman, Talia (Utah Valley University)
Faculty Advisor: Zahn, Geoffrey (Utah Valley University, Biology)

We assessed the ability of Pleurotus ostreatus, Oyster mushroom, to efficiently decompose waste products containing cellulose. As common pollutants are comprised of cellulose it is important to understand organisms that have cellulolytic enzymes such as P. ostreatus. The fungus P. ostreatus was introduced to the substrates of paper textiles, peanut shells, and livestock manure. After each treatment, the yield of mushroom production and remaining substrate was measured. As these substrates have been broken down during mushroom production they can be sold as inorganic fertilizer or compost. As well, Oyster mushrooms can be purchased and enjoyed in meals. Mushroom cultivation is a multi-billion dollar industry, and excessive wastes are a strain on the environment. By understanding what cellulase substrates produce the greatest yields we can eliminate waste all while increasing profits. Oh, and eating great mushrooms!
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