Agriculture

Waldron, Connor; Moley, Laura; Isom, S. Clay (Utah State University)
Faculty Advisor: Isom, S. Clay (College of Agriculture and Applied Sciences; Animal, Dairy, and Veterinary Sciences Department)

In vitro maturation (IVM) is a process that immature oocytes undergo before in vitro fertilization is performed. During IVM, immature oocytes are extracted through aspiration from follicles and receive the necessary hormones to resume meiosis in culture. Success rate of IVM is significantly lower compared to in vivo maturation of oocytes. Oocytes selected for IVM are extracted from small ovarian follicles (1-3mm), which are 4-6 weeks away from complete maturation and subsequent ovulation. During that period, the DNA of the oocyte is undergoing the process of DNA methylation, the addition of a methyl group to cytosines within a CpG context. DNA methylation is an epigenetic change that causes modification to gene expression where methylated gene promoters turn off gene expression. Proper oocyte gene expression is very important in the development of a healthy embryo. Incomplete methylation of aspirated oocytes may be another factor contributing to the low success rates of IVM. We hypothesize that there is a difference in the amount of methylation between oocytes from small ovarian follicles that are further from natural ovulation and oocytes from large ovarian follicles which are developmentally closer to ovulation, with higher levels of methylation in oocytes from large ovarian follicles. To test our hypothesis, large porcine ovarian follicles (7mm and larger) and small porcine ovarian follicles (1-3mm) will be aspirated for ovaries. The oocytes will be stained the two stains, one to visualize the nucleus of the oocyte indicating the presence of DNA and the other to visualize DNA methylation. Fluorescent images will be taken of the oocytes, and the small and large follicle oocyte groups will be compared for genome wide methylation levels.

Colbert, Jeffrey (Weber State University)
Faculty Advisor: Schramm, Katherina (Weber State University, Botany); Hillbig, Bridgette (Weber State University, Botany); Walker, Edward (Weber State University, Chemistry)

Hemp is a multimillion-dollar industry; however, it is only grown in a few states in the US. In prehistoric time, there was a brackish lake that covered the valley of Utah�s Wasatch Front. Because of this lake and different drainage events, the soil chemistry and composition changed depending on the depth and salts concentration of the lake.
This study tested soils from three different locations along the Wasatch Front for their potential to support healthy growth of hemp plants. Mg, Ca, N, P, K concentrations were tested along with pH in soils from different geological strata. In an outdoor environment hemp was grown in five-gallon containers to determine if those soils will support hemp growth yielding high fiber content and quality. Potting soil was used as a control. The same soil characterization tests were conducted after the plants were harvested to show what amendments are needed for a successful crop in following seasons. Mature plants� inflorescences were tested for tetrahydrocannabinol (THC), and cannabidiol (CBD) levels by Utah Department of Agriculture and Food (UDAF). All plants contained less than 0.3% THC. Fiber quantity was measured using confocal microscopy techniques and compared against the different soil collection locations. Water retting was conducted to extract fiber from stalks. Thin Layer Chromatography (TLC) was conducted confirming UDAF�s findings of THC and CBD.